Lactobacillus spp. reduces morphological changes and oxidative stress induced by deoxynivalenol on the intestine and liver of broilers.
Identifieur interne : 000146 ( Main/Exploration ); précédent : 000145; suivant : 000147Lactobacillus spp. reduces morphological changes and oxidative stress induced by deoxynivalenol on the intestine and liver of broilers.
Auteurs : Marielen De Souza [Brésil] ; Ana Angelita S. Baptista [Brésil] ; Milton J J. Valdiviezo [Brésil] ; Larissa Justino [Brésil] ; Maísa F. Menck-Costa [Brésil] ; Camila R. Ferraz [Brésil] ; Eduardo M. Da Gloria [Brésil] ; Waldiceu A. Verri [Brésil] ; Ana Paula F R L. Bracarense [Brésil]Source :
- Toxicon : official journal of the International Society on Toxinology [ 1879-3150 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Poulets.
- physiologie : Stress oxydatif.
- toxicité : Mycotoxines, Trichothécènes.
- Aliment pour animaux, Animaux, Contamination des aliments, Intestins, Lactobacillus.
English descriptors
- KwdEn :
- MESH :
- chemical , toxicity : Mycotoxins, Trichothecenes.
- microbiology : Chickens.
- physiology : Oxidative Stress.
- Animal Feed, Animals, Food Contamination, Intestines, Lactobacillus.
Abstract
The mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide, frequently resulting in poor performance and economic losses. Data concerning the effects on poultry health or focusing on intestinal toxicity or the response to oxidative stress are scarce. Also, there is a need for strategies to mitigate the negative effects of DON. This study aimed to investigate the effects of Lactobacillus spp. treatments on the intestine, liver and kidney of poultry fed a DON-contaminated diet. To achieve this aim, histological, morphometrical and histochemical assays were performed. The oxidative stress response was also analyzed by the tests: reduced glutathione, ferric reducing ability, reducing of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), nitro blue tetrazolium detection of superoxide anion, and thiobarbituric acid reactive substances. One-day-old broilers chickens (n 50) were submitted to the following treatments: control, DON (19.3 mg kg-1), viable Lactobacillus spp. + DON (VL + DON), heat-inactivated Lactobacillus spp. + DON (HIL + DON), Lactobacillus spp. culture supernatant + DON (LCS + DON). The animals received the contaminated diet for seven days. DON increased the intestinal and liver lesion score, while the Lactobacillus spp. treatments (LT) remained like the control. DON reduced the villi height and increased the crypt depths. The LT showed crypt depths similar to control, and higher villi: crypt ratio in duodenum and jejunum. In the ileum, the LT reduced the goblet cell count in relation to DON group. DON increased the number of intraepithelial lymphocytes (IEL) in jejunum and ileum, while the VL + DON treatment induced a significant decrease in IEL in comparison to DON. DON-diet induced an oxidative stress response in the intestine and liver, and also reduced the antioxidant capacity in these tissues, while LT treatments remained mostly similar to control. DON induced no change in redox balance in the kidney. The LT improved the intestinal health after DON acute exposure, reducing the oxidative stress damage mainly on jejunum and liver.
DOI: 10.1016/j.toxicon.2020.07.002
PubMed: 32687887
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Valdiviezo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970</wicri:regionArea><wicri:noRegion>86057-970</wicri:noRegion></affiliation></author><author><name sortKey="Justino, Larissa" sort="Justino, Larissa" uniqKey="Justino L" first="Larissa" last="Justino">Larissa Justino</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Avian Medicine, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Avian Medicine, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970</wicri:regionArea><wicri:noRegion>86057-970</wicri:noRegion></affiliation></author><author><name sortKey="Menck Costa, Maisa F" sort="Menck Costa, Maisa F" uniqKey="Menck Costa M" first="Maísa F" last="Menck-Costa">Maísa F. Menck-Costa</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Avian Medicine, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Avian Medicine, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970</wicri:regionArea><wicri:noRegion>86057-970</wicri:noRegion></affiliation></author><author><name sortKey="Ferraz, Camila R" sort="Ferraz, Camila R" uniqKey="Ferraz C" first="Camila R" last="Ferraz">Camila R. Ferraz</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Pain, Inflammation, Neuropathy and Cancer, Universidade Estadual de Londrina, Londrina, Paraná, 86057-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Pain, Inflammation, Neuropathy and Cancer, Universidade Estadual de Londrina, Londrina, Paraná, 86057-970</wicri:regionArea><wicri:noRegion>86057-970</wicri:noRegion></affiliation></author><author><name sortKey="Da Gloria, Eduardo M" sort="Da Gloria, Eduardo M" uniqKey="Da Gloria E" first="Eduardo M" last="Da Gloria">Eduardo M. Da Gloria</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Mycology, Universidade de São Paulo, São Paulo, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Mycology, Universidade de São Paulo, São Paulo</wicri:regionArea><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Verri, Waldiceu A" sort="Verri, Waldiceu A" uniqKey="Verri W" first="Waldiceu A" last="Verri">Waldiceu A. Verri</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Pain, Inflammation, Neuropathy and Cancer, Universidade Estadual de Londrina, Londrina, Paraná, 86057-970, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Pain, Inflammation, Neuropathy and Cancer, Universidade Estadual de Londrina, Londrina, Paraná, 86057-970</wicri:regionArea><wicri:noRegion>86057-970</wicri:noRegion></affiliation></author><author><name sortKey="Bracarense, Ana Paula F R L" sort="Bracarense, Ana Paula F R L" uniqKey="Bracarense A" first="Ana Paula F R L" last="Bracarense">Ana Paula F R L. Bracarense</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil. Electronic address: anapaula@uel.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970</wicri:regionArea><wicri:noRegion>86057-970</wicri:noRegion></affiliation></author></analytic><series><title level="j">Toxicon : official journal of the International Society on Toxinology</title><idno type="eISSN">1879-3150</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal Feed (MeSH)</term><term>Animals (MeSH)</term><term>Chickens (microbiology)</term><term>Food Contamination (MeSH)</term><term>Intestines (MeSH)</term><term>Lactobacillus (MeSH)</term><term>Mycotoxins (toxicity)</term><term>Oxidative Stress (physiology)</term><term>Trichothecenes (toxicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aliment pour animaux (MeSH)</term><term>Animaux (MeSH)</term><term>Contamination des aliments (MeSH)</term><term>Intestins (MeSH)</term><term>Lactobacillus (MeSH)</term><term>Mycotoxines (toxicité)</term><term>Poulets (microbiologie)</term><term>Stress oxydatif (physiologie)</term><term>Trichothécènes (toxicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Mycotoxins</term><term>Trichothecenes</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Poulets</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Chickens</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Stress oxydatif</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Oxidative Stress</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Mycotoxines</term><term>Trichothécènes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animal Feed</term><term>Animals</term><term>Food Contamination</term><term>Intestines</term><term>Lactobacillus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Aliment pour animaux</term><term>Animaux</term><term>Contamination des aliments</term><term>Intestins</term><term>Lactobacillus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide, frequently resulting in poor performance and economic losses. Data concerning the effects on poultry health or focusing on intestinal toxicity or the response to oxidative stress are scarce. Also, there is a need for strategies to mitigate the negative effects of DON. This study aimed to investigate the effects of Lactobacillus spp. treatments on the intestine, liver and kidney of poultry fed a DON-contaminated diet. To achieve this aim, histological, morphometrical and histochemical assays were performed. The oxidative stress response was also analyzed by the tests: reduced glutathione, ferric reducing ability, reducing of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), nitro blue tetrazolium detection of superoxide anion, and thiobarbituric acid reactive substances. One-day-old broilers chickens (n 50) were submitted to the following treatments: control, DON (19.3 mg kg<sup>-1</sup>), viable Lactobacillus spp. + DON (VL + DON), heat-inactivated Lactobacillus spp. + DON (HIL + DON), Lactobacillus spp. culture supernatant + DON (LCS + DON). The animals received the contaminated diet for seven days. DON increased the intestinal and liver lesion score, while the Lactobacillus spp. treatments (LT) remained like the control. DON reduced the villi height and increased the crypt depths. The LT showed crypt depths similar to control, and higher villi: crypt ratio in duodenum and jejunum. In the ileum, the LT reduced the goblet cell count in relation to DON group. DON increased the number of intraepithelial lymphocytes (IEL) in jejunum and ileum, while the VL + DON treatment induced a significant decrease in IEL in comparison to DON. DON-diet induced an oxidative stress response in the intestine and liver, and also reduced the antioxidant capacity in these tissues, while LT treatments remained mostly similar to control. DON induced no change in redox balance in the kidney. The LT improved the intestinal health after DON acute exposure, reducing the oxidative stress damage mainly on jejunum and liver.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32687887</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-3150</ISSN><JournalIssue CitedMedium="Internet"><Volume>185</Volume><PubDate><Year>2020</Year><Month>Oct</Month><Day>15</Day></PubDate></JournalIssue><Title>Toxicon : official journal of the International Society on Toxinology</Title><ISOAbbreviation>Toxicon</ISOAbbreviation></Journal><ArticleTitle>Lactobacillus spp. reduces morphological changes and oxidative stress induced by deoxynivalenol on the intestine and liver of broilers.</ArticleTitle><Pagination><MedlinePgn>203-212</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0041-0101(20)30306-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.toxicon.2020.07.002</ELocationID><Abstract><AbstractText>The mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide, frequently resulting in poor performance and economic losses. Data concerning the effects on poultry health or focusing on intestinal toxicity or the response to oxidative stress are scarce. Also, there is a need for strategies to mitigate the negative effects of DON. This study aimed to investigate the effects of Lactobacillus spp. treatments on the intestine, liver and kidney of poultry fed a DON-contaminated diet. To achieve this aim, histological, morphometrical and histochemical assays were performed. The oxidative stress response was also analyzed by the tests: reduced glutathione, ferric reducing ability, reducing of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), nitro blue tetrazolium detection of superoxide anion, and thiobarbituric acid reactive substances. One-day-old broilers chickens (n 50) were submitted to the following treatments: control, DON (19.3 mg kg<sup>-1</sup>), viable Lactobacillus spp. + DON (VL + DON), heat-inactivated Lactobacillus spp. + DON (HIL + DON), Lactobacillus spp. culture supernatant + DON (LCS + DON). The animals received the contaminated diet for seven days. DON increased the intestinal and liver lesion score, while the Lactobacillus spp. treatments (LT) remained like the control. DON reduced the villi height and increased the crypt depths. The LT showed crypt depths similar to control, and higher villi: crypt ratio in duodenum and jejunum. In the ileum, the LT reduced the goblet cell count in relation to DON group. DON increased the number of intraepithelial lymphocytes (IEL) in jejunum and ileum, while the VL + DON treatment induced a significant decrease in IEL in comparison to DON. DON-diet induced an oxidative stress response in the intestine and liver, and also reduced the antioxidant capacity in these tissues, while LT treatments remained mostly similar to control. DON induced no change in redox balance in the kidney. The LT improved the intestinal health after DON acute exposure, reducing the oxidative stress damage mainly on jejunum and liver.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>de Souza</LastName><ForeName>Marielen</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil; Laboratory of Avian Medicine, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baptista</LastName><ForeName>Ana Angelita S</ForeName><Initials>AAS</Initials><AffiliationInfo><Affiliation>Laboratory of Avian Medicine, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Valdiviezo</LastName><ForeName>Milton J J</ForeName><Initials>MJJ</Initials><AffiliationInfo><Affiliation>Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Justino</LastName><ForeName>Larissa</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laboratory of Avian Medicine, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Menck-Costa</LastName><ForeName>Maísa F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Laboratory of Avian Medicine, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ferraz</LastName><ForeName>Camila R</ForeName><Initials>CR</Initials><AffiliationInfo><Affiliation>Laboratory of Pain, Inflammation, Neuropathy and Cancer, Universidade Estadual de Londrina, Londrina, Paraná, 86057-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>da Gloria</LastName><ForeName>Eduardo M</ForeName><Initials>EM</Initials><AffiliationInfo><Affiliation>Laboratory of Mycology, Universidade de São Paulo, São Paulo, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Verri</LastName><ForeName>Waldiceu A</ForeName><Initials>WA</Initials><Suffix>Jr</Suffix><AffiliationInfo><Affiliation>Laboratory of Pain, Inflammation, Neuropathy and Cancer, Universidade Estadual de Londrina, Londrina, Paraná, 86057-970, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bracarense</LastName><ForeName>Ana Paula F R L</ForeName><Initials>APFRL</Initials><AffiliationInfo><Affiliation>Laboratory of Animal Pathology, Universidade Estadual de Londrina, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná, 86057-970, Brazil. Electronic address: anapaula@uel.br.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Toxicon</MedlineTA><NlmUniqueID>1307333</NlmUniqueID><ISSNLinking>0041-0101</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009183">Mycotoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014255">Trichothecenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>JT37HYP23V</RegistryNumber><NameOfSubstance UI="C007262">deoxynivalenol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000821" MajorTopicYN="N">Animal Feed</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005506" MajorTopicYN="N">Food Contamination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007422" MajorTopicYN="N">Intestines</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007778" MajorTopicYN="N">Lactobacillus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009183" MajorTopicYN="N">Mycotoxins</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014255" MajorTopicYN="N">Trichothecenes</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Detoxification</Keyword><Keyword MajorTopicYN="N">Gut health</Keyword><Keyword MajorTopicYN="N">Mycotoxins</Keyword><Keyword MajorTopicYN="N">Poultry</Keyword><Keyword MajorTopicYN="N">Probiotic</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>06</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32687887</ArticleId><ArticleId IdType="pii">S0041-0101(20)30306-8</ArticleId><ArticleId IdType="doi">10.1016/j.toxicon.2020.07.002</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><country name="Brésil"><noRegion><name sortKey="De Souza, Marielen" sort="De Souza, Marielen" uniqKey="De Souza M" first="Marielen" last="De Souza">Marielen De Souza</name></noRegion><name sortKey="Baptista, Ana Angelita S" sort="Baptista, Ana Angelita S" uniqKey="Baptista A" first="Ana Angelita S" last="Baptista">Ana Angelita S. Baptista</name><name sortKey="Bracarense, Ana Paula F R L" sort="Bracarense, Ana Paula F R L" uniqKey="Bracarense A" first="Ana Paula F R L" last="Bracarense">Ana Paula F R L. Bracarense</name><name sortKey="Da Gloria, Eduardo M" sort="Da Gloria, Eduardo M" uniqKey="Da Gloria E" first="Eduardo M" last="Da Gloria">Eduardo M. Da Gloria</name><name sortKey="Ferraz, Camila R" sort="Ferraz, Camila R" uniqKey="Ferraz C" first="Camila R" last="Ferraz">Camila R. Ferraz</name><name sortKey="Justino, Larissa" sort="Justino, Larissa" uniqKey="Justino L" first="Larissa" last="Justino">Larissa Justino</name><name sortKey="Menck Costa, Maisa F" sort="Menck Costa, Maisa F" uniqKey="Menck Costa M" first="Maísa F" last="Menck-Costa">Maísa F. Menck-Costa</name><name sortKey="Valdiviezo, Milton J J" sort="Valdiviezo, Milton J J" uniqKey="Valdiviezo M" first="Milton J J" last="Valdiviezo">Milton J J. Valdiviezo</name><name sortKey="Verri, Waldiceu A" sort="Verri, Waldiceu A" uniqKey="Verri W" first="Waldiceu A" last="Verri">Waldiceu A. Verri</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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